Ciforadenant

Inhibition of the adenosine A2a receptor modulates expression of T cell coinhibitory receptors and improves effector function for enhanced checkpoint blockade and ACT in murine cancer models

Robert D. Leone1 · Im‑Meng Sun1 · Min‑Hee Oh1 · Im‑Hong Sun1 · Jiayu Wen1 · Judson Englert1,2 · Jonathan D. Powell3

Received: 23 January 2018 / Accepted: 8 June 2018
© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Abstract

Adenosine signaling via the A2a receptor (A2aR) is emerging as an important checkpoint of immune responses. The pres- ence of adenosine in the inflammatory milieu or generated by the CD39/CD73 axis on tissues or T regulatory cells serves to regulate immune responses. By nature of the specialized metabolism of cancer cells, adenosine levels are increased in the tumor microenvironment and contribute to tumor immune evasion. To this end, small molecule inhibitors of the A2aR are being pursued clinically to enhance immunotherapy. Herein, we demonstrate the ability of the novel A2aR antagonist, CPI-444, to dramatically enhance immunologic responses in models of checkpoint therapy and ACT in cancer. Furthermore, we demonstrate that A2aR blockade with CPI-444 decreases expression of multiple checkpoint pathways, including PD-1 and LAG-3, on both CD8+ effector T cells (Teff) and FoxP3+ CD4+ regulatory T cells (Tregs). Interestingly, our studies demonstrate that A2aR blockade likely has its most profound effects during Teff cell activation, significantly decreasing PD-1 and LAG-3 expression at the draining lymph nodes of tumor bearing mice. In contrast to previous reports using A2aR knockout models, pharmacologic blockade with CPI-444 did not impede CD8 T cell persistence or memory recall. Overall these findings not only redefine our understanding of the mechanisms by which adenosine inhibits immunity but also have important implications for the design of novel immunotherapy regimens.

Keywords : Immunotherapy · Immune checkpoint · A2a · PD-1 · Lag-3 · Treg

Introduction

Initially described in seminal studies by the Sitkovsky group, adenosine signaling through the A2a receptor on immune cells is a critical regulator of inflammation and immune response [1]. Findings from this initial work demonstrated that pharmacologic or genetic blockade of the A2a recep- tor greatly enhanced inflammation in the setting of an anti- pathogen immune response. These early studies were criti- cal in prompting investigations of the role of the A2aR in modulating immune responses in cancer. As such, subse- quent work by the Sitkovsky group demonstrated that A2aR signaling on antitumor T cells inhibits effector responses and protects tumor development [2]. This group was the first to report the therapeutic application of pharmacologic A2aR blockade in enhancing antitumor immune responses and improving tumor rejection in murine models of melanoma. Interestingly, tumor responses were shown to be secondary to both direct CD8+ T cell killing, as well as through A2aR blockade-mediated inhibition of neovascularization within the TME [2]. Subsequent studies from our group and others confirmed and extended the work of the Sitkovsky group by successfully applying A2aR blockade in combination with anti-PD-1 inhibition to antitumor regimens in murine models [3–5]. Work by Stagg and Smyth has elucidated the importance of adenosine-A2aR signaling in triple nega- tive breast cancer metastasis and response to chemother- apy [6–9]. Further broadening the understanding of A2aR signaling in immune regulation, our group has previously demonstrated the importance of A2aR to the generation of FoxP3+ LAG-3+ regulatory T cells [10].

The importance of adenosine signaling as an immune regulatory mechanism for tumor evasion is underscored by the high levels of adenosine generated within the TME. Cancer cells are metabolically reprogrammed to meet the enormous biosynthetic demands that accompany rapid and continuous proliferation. In addition to supporting tumor growth, this specialized metabolism serves to greatly alter the TME, making it hypoxic, acidic, and depleted of nutri- ents [11, 12]. Adenosine generation is a direct consequence of profound hypoxia and high rates of cellular turnover in tumors [13–15]. Specifically, adenosine is generated through release of intracellular adenosine from tumor cells, as well as through the enzymatic degradation of extracellular ATP by the hypoxia-responsive ectonucleotidases CD39 and CD73, which are often highly expressed on a variety of cells within the TME [16–19].

Herein, we present initial preclinical immunotherapy data using the novel A2aR inhibitor CPI-444, which is cur- rently being tested in a phase 1b clinical trial [20]. Using this inhibitor, we also present data refining the role of adenosine in modulating T cell responses in cancer. First, our studies show that inhibiting adenosine signaling through the A2a receptor suppresses a range of distinct checkpoint receptors (including PD-1 and LAG-3) on both Teff cells and Tregs. In lowering checkpoint expression on T cells, the threshold for productive anti-PD-1 therapy is lowered, resulting in a syn- ergistic response with CPI-444 and anti-PD-1 combination therapy. Notably, the modulation of checkpoint pathways on Teff cells in these studies is a phenomenon detected primar- ily at tumor-draining lymph nodes. In analagous findings, we report that CPI-444-treated mice receiving activated antitu- mor T cells show enhanced antitumor responses in mouse models of ACT. These findings in distinct immunotherapy models suggest an especially prominent role for A2aR blockade during initial Teff priming, which may underlie the marked results in combination with PD-1 blockade in our models. Our work also describes previously unreported effects of A2aR blockade on Tregs within the TME. In this regard, we report suppression of FoxP3 expression, as well as downregulation of a range of immune checkpoint path- ways, including PD-1 and LAG-3. Lastly, we show enhanced effector function of Teff cells within the TME in the set- ting of CPI-444 monotherapy, as well as in combination with ACT. Overall, in addition to demonstrating the abil- ity of CPI-444 to significantly enhance antitumor immune responses in a variety of immunotherapy regimens, we define novel adenosine-mediated mechanisms that promote tumor immune evasion.

Methods
Antibodies and reagents

Antibodies against the following proteins were purchased from BD Biosciences: CD4 (RM4-5), CD8α (53-6.7), CD69 (H1.2F3), CD90.1 (OX-7), CD44 (IM7), IL-2 (JES6-5H4),TNF-α (MP6-XT22), and IFNγ (XMG1.2). Antibodies against the following proteins were purchased from eBiosci- ence: PD-1 (RMP-130), LAG-3 (C9B7W), TIM-3 (RMT3- 23, 8B.2C12), CTLA-4 (UC10-4B9), FoxP3 (FJK-16s), Ki67 (SolA15), 41-BB (17B5), CD62L (MEL-14), CD127 (A7R34), T-bet (eBio4B10), and granzyme B (GB11). Other reagents used included OVA class-I tetramer (H-2 kb/ SIINFEKL, MBL) and OVA class-I peptide (SIINFEKL, AnaSpec). GolgiPlug or GolgiStop (BD Biosciences) was used to inhibit cytokine secretion, and the BD Cytofix/ Cytoperm Kit (BD Biosciences) was used for intracellular staining of cytokines. The FoxP3 Fixation/Permeabilization Kit (eBioscience) was used for transcription factor staining.

T cell ex vivo stimulation

T cells were stimulated in the presence of GolgiStop for 4 h at 37 °C with either 10 µg/ml OVA class-I peptide (SIIN- FEKL) or PMA (50 ng/ml) and Ionomycin (500 ng/ml).

Flow cytometry

All experiments were performed on a BD FACSCalibur or BD Celesta and analyzed using FlowJo software (FlowJo, LLC). For all flow cytometry experiments, gates were set appropriately with unstimulated and isotype controls.

Vaccinia infection and Listeria rechallenge

C57BL/6 mice were infected with 1 × 106 PFU vaccinia- OVA (made in-house) by retro-orbital (r.o.) injection. Cheek bleeds were performed at indicated time points for analyses during acute response. Distinct cohorts of mice received daily administration of vehicle or 1–10 mg/ kg CPI-444 (Corvus) by gavage from day 1 to 5. Mice received a secondary infection with 2 × 106 CFU of OVA- expressing Listeria monocytogenes (DactA, Din1B) (LM- OVA) (i.p.) (gift from Aduro Biotech) on day 30. Mice were sacrificed 6 days after secondary infection, and splenocytes were isolated for analysis.

Tumor experiments

Unless otherwise noted, all tumor injections were admin- istered on the right flank. For the MC38 model, C57BL/6 WT mice were injected with 5 × 105 MC38 cells (s.c.) cultured in DMEM-based media. For the CT26 model, BALB/c mice were injected with 5 × 105 CT26 cells (s.c.) cultured in RPMI-based media. CPI-444 is sup- plied in 40% hydroxypropyl beta-cyclodextrin which was administered for all vehicle-treated control experiments. Mice were treated with CPI-444 (Corvus) or vehicle by daily gavage at indicated times and concentrations. Mice were randomized based on tumor size before initiating anti-PD-1 therapy or transfer of activated OT1 cells for adoptive transfer experiments. Anti-PD-1 mAb (RMP1- 14, Bioxcell) was administered by i.p. injection (100 ug/ mouse) at indicated time points. For the OVA-expressing B16 melanoma model, C57BL/6 WT mice received a s.c. injection of 2 × 105 B16-OVA melanoma cells (gift of Hyam Levitsky) cultured under OVA selection media con- taining 400 µg/ml G418 (Life technologies). Seven days after tumor injection, mice received an adoptive transfer of 1.5 × 106 activated OT1 cells derived from splenocytes, which had been stimulated in vitro with SIINFEKL pep- tide for 48 h, expanded in IL-2 (1 ng/mL) for 24 h and iso- lated with Ficol gradient centrifugation. Mice were treated with CPI-444 or vehicle by gavage daily at indicated times and concentrations administered in 200 µL volume. Tumor burden was assessed every 2–4 days by measuring length and width of tumor. Tumor volume was calculated using the formula V = (L × W × W)/2, where V is tumor volume, W is tumor width, and L is tumor length. Mice were sacri- ficed when tumor reached 2 cm in any dimension, became ulcerate or necrotic, or caused functional deficits.

TIL isolation

Tumors were harvested from mice at indicated time points. Explanted tumors were manually disrupted before incubat- ing in collagenase type I (Gibco) and DNase in RPMI for 30 min at 37 °C. Tumor mixtures, spleens, and nondraining (left inguinal) and draining (right inguinal) lymph nodes were dissociated through a 70-µm filter and washed with PBS. Splenocytes and blood were treated with ACK (Qual- ity Biological) lysing buffer and washed with PBS before staining for flow cytometry.

Cell lines

MC38 cells were donated by CORVUS pharmaceuticals. B16-OVA melanoma cells were a gift from Hyam Levit- sky. All other tumor cell lines used were obtained from the ATCC.

Statistics

All graphs were created using GraphPad Prism software, and statistical analyses were calculated using GraphPad Prism. Comparisons between two independent groups were assessed by either Student’s t test or Mann–Whitney t tests. A p value of less than 0.05 was considered statistically significant.

Results

CPI‑444 suppresses checkpoint pathways, enhances initial immune response, and augments memory response to viral infection

CPI-444 is an oral small molecule inhibitor of A2aR [21] which has demonstrated high selectivity and ability to block A2aR in in vitro studies. To establish the effects of CPI-444 during in vivo immune responses, we initially assessed its effect on CD8+ T cell responses to viral challenge. Follow- ing immunization with vaccinia-OVA, mice were treated with vehicle or CPI-444, and the CD8+T cell response was monitored in the peripheral blood. At the peak of response, flow cytometric analysis using tetramer staining revealed a significant enhancement in the antigen-specific CD8+ (Tet-OVA+) T cell response in mice treated with CPI-444 (Fig. 1a, b). Furthermore, antigen-specific CD8+ T cells in CPI-444-treated mice had decreased expression of immune checkpoint surface receptors, including PD-1, TIM-3, and LAG-3 (Fig. 1c–e). Interestingly, decreased checkpoint receptor expression, including PD-1, LAG-3, TIM-3, and CTLA-4 was also noted on Tregs (Fig. 1f, Supplemental Fig. 1). Tregs in CPI-444-treated mice also showed lower expression of the lineage-defining transcription factor FoxP3 (Fig. 1g).

Fig. 1 A2aR blockade with CPI-444 modulates checkpoint expres- sion and enhances acute immune response to vaccine. C57BL/6 mice immunized with vaccinia-OVA (106 PFU) by retro-orbital (r.o.) injection received either vehicle or CPI-444 (10 mg/kg) p.o. daily for 5 days starting 1 day post-infection. a Flow cytometry showing time course of Tet-OVA+T cell response monitored by cheek bleeding using tetramer-OVA staining. b Data summary plot for Tet-OVA+ T cell response on day 7 post-infection. c–e Flow cytometric analyses of PD-1 (c), TIM-3 (d), and LAG-3 (e) expression on Tet-OVA+ T cells harvested on day 7 (PD-1) and day 11 (TIM-3, LAG-3) are shown as representative MFI histograms (left) and summation data plots (right). f–g Data summary plots from flow cytometry analyses of PD-1, TIM-3, LAG-3 (f) expression, and FoxP3 (g) expression on Tregs in the peripheral blood on day 11 post-infection. Error bars represent SEM. Data are representative of three independent experi- ments with n = 4–5 per group. *p < 0.05, **p < 0.01 using two-tailed Student’s t test. To assess the effects of CPI-444 on immune memory response, mice were rechallenged with OVA-expressing Listeria monocytogenes (LM-OVA) 30 days after initial vac- cinia-OVA vaccination. Despite receiving no further treat- ment during rechallenge, mice treated with CPI-444 during initial vaccinia-OVA exposure showed a significant enhance- ment in recall response (Fig. 2a). Interestingly, as in the case of the acute viral response, Tet-OVA+ T cells responding to LM-OVA rechallenge showed reduced expression of checkpoint receptors PD-1, TIM-3, and LAG-3 (Fig. 2b). Furthermore, Tet-OVA+ T cells from mice treated with CPI-444 showed increased ability to produce TNFα, IFNγ (Fig. 2c), and IL-2 (Fig. 2d). Previous studies had reported decreased T cell persistence and decreased CD127 expres- sion in A2a-deleted naïve and memory T cells [22, 23]. In our studies, while antigen-specific T cells did show a slight trend toward decreased expression of CD127 during initial vaccine response (Supplemental Fig. 2), memory response of TetOVA+ CD8+ T cells was not suppressed in mice that received CPI-444 during initial vaccination (Fig. 2a). Our data demonstrate that pharmacologic inhibition of A2a receptor with CPI-444 during initial CTL priming increases the magnitude of T cell response and suppresses immune checkpoint receptor expression during both initial vacci- nation and subsequent challenge. Furthermore, CPI-444 treatment during initial vaccination significantly enhances effector function of memory CD8+ T cells upon LM-OVA rechallenge. CPI‑444 monotherapy modestly suppresses tumor growth and improves survival in mouse tumor models Our initial application of CPI-444 to tumor immunotherapy evaluated its effect as monotherapy in MC38 and CT26 colon murine cancer models. As shown in Supplemental Fig. 3a–c, daily CPI-444 treatment resulted in modest but significant reduction in tumor growth and survival benefit in the MC38 model. A trend toward reduced tumor growth in mice receiving daily CPI-444 was also noted in the CT26 model (Supplemental Fig. 3d, e). Overall our findings show that CPI-444 treatment as a single agent results in modest suppression of tumor growth in mouse tumor models. A2aR blockade with CPI‑444 enhances anti‑PD‑1 immunotherapy Recent studies have reported that decreased expression of PD-1 on antitumor T cells can lower the threshold for pro- ductive anti-PD-1 immunotherapy [24]. Given our findings demonstrating suppression of PD-1 expression on anti-viral Teff cells (Fig. 1), we were interested in combining CPI-444 with anti-PD-1 therapy. We titrated the dose and frequency of anti-PD-1 mAb as a single agent to yield a small but measurable effect on tumor control (10–20% cure rate in the CT26 tumor model). Strikingly, anti-PD-1 and CPI-444 combination treatment demonstrated a dramatic improve- ment in tumor regression and animal survival in both CT26 (Fig. 3a–c) and MC38 (Fig. 3d–f) tumor models. The effect was particularly marked in the CT26 tumor model, wherein combination therapy increased the complete rejection rate from 20% in mice receiving anti-PD-1 alone to 70% in mice treated with combined CPI-444 and anti-PD-1 (Fig. 3a–c). Our data indicate that using CPI-444 in combination with anti-PD-1 therapy can lead to dramatic improvement in antitumor immune response over either agent used as monotherapy. A2aR blockade with CPI‑444 suppresses PD‑1 and LAG‑3 checkpoint pathways on CD8+T cells and Tregs in tumor bearing mice. The marked enhancement of anti-PD-1 therapy with CPI- 444 in the CT26 model (Fig. 3a-c) was particularly interest- ing in light of the modest effect of CPI-444 monotherapy (Supplemental Fig. 3d, e). As such, we wanted to understand how A2aR inhibition conditions T cells during anti-PD-1 therapy and whether the expression of checkpoint receptors on Teff cells is affected. CT26 bearing mice were treated with vehicle or CPI-444. Tumor, spleen, draining, and non- draining lymph nodes were harvested after 14 days of treat- ment and assessed by flow cytometry (Fig. 4a). Infiltrating Teff cells within tumors or nondraining lymph nodes (ndLN) of mice treated with CPI-444 did not demonstrate decreased expression of PD-1 (Fig. 4b, c) or LAG-3 (data not shown) compared to vehicle-treated mice. Interestingly, however, we found a significant suppression of PD-1 and LAG-3 expression on activated CD8+ CD44+ T cells within tumor- draining lymph nodes (dLN) (Fig. 4d), with PD-1 expression also decreased on splenic CD8+ CD44+ T cells (Fig. 4e). Our results demonstrate a marked influence of adenosine signaling on Teff cells at draining lymph nodes, which, when blocked by CPI-444 treatment leads to suppression of check- point pathways. Tregs play an important role in regulating antitumor immune responses. Like Teff cells, Tregs have been shown to be influenced by checkpoint pathways [25, 26]. We sought to evaluate the effects of A2aR blockade on Tregs within the TME. In our model, A2aR blockade with CPI-444 had pronounced effects on coinhibitory receptor expression on Tregs as well (Fig. 4f). Analogous to changes on Teff cells, PD-1 and LAG-3 expressions were significantly decreased on tumor-infiltrating Tregs in CPI-444-treated mice. FoxP3 expression in tumor-infiltrating Tregs was also suppressed in CPI-444-treated mice (Fig. 4g). Overall, our results demon- strate a broad attenuating effect on suppressive pathways on both effector and regulatory T cells, with significant effects on Teff cells within draining lymph nodes and on Tregs within the TME. ◂Fig. 2 A2aR blockade with CPI-444 during initial vaccination enhances Tet-OVA+T cell memory response. Without further treat- ment, mice from Fig. 1 were rechallenged with OVA-expressing Listeria monocytogenes (LM-OVA; 106 CFU, r.o.) 30 days after ini- tial vaccina-OVA immunization. Splenocytes were harvested 6 days post-LM-OVA rechallenge for analysis. a Flow cytometry showing Tet-OVA+T cell response. b Flow cytometric analyses of checkpoint expression on Tet-OVA+T cells are shown as representative MFI histograms (left) and summation data plots (right). c-d Flow cytom- etry analyses of the fraction of cytokine production of CD8+ sple- nocytes upon peptide stimulation is shown. Fraction of Tet-OVA+ T cells producing IFNγ, TNFα, double positive IFNγ+TNFα+ (c), and IL-2 (d) is shown. Error bars represent SEM. Data are representative of three independent experiments with n = 4–5 per group. *p < 0.05,**p < 0.01, ***p < 0.001 using two-tailed Student’s t test. A2aR blockade with CPI‑444 enhances effector phenotype and function of tumor‑infiltrating CD8+ T cells We were interested in assessing the functional consequences of our findings within the CT26 tumor TME. Evaluation of transcription factors by flow cytometry revealed increased expression of T-bet on infiltrating CD8+ T cells (Fig. 4h), as well as increased expression of the activation marker 41-BB (CD137) within the TME (Fig. 4h). Upon ex vivo stimula- tion with PMA and ionomycin, tumor-infiltrating Teff from CPI-444-treated mice showed significantly increased frac- tion of TNFα+ CD8+T cells, IFNγ+ CD8+T cells, as well as TNFα+IFNγ+ double positive CD8+ T cells (Fig. 4i). These results demonstrate that A2aR blockade through CPI-444 monotherapy enhances effector function of tumor- infiltrating CD8+ T cells. CPI‑444 enhances ACT in the B16‑OVA tumor model Given our findings demonstrating the impact of A2aR block- ade on T cell expansion and effector function following vac- cination (Figs. 1, 2), we were interested in applying CPI-444 to a model of adoptive cell therapy. Mice with OVA-express- ing B16 tumors received 1.5 million activated OVA trans- genic (OT1) CD8+ T cells. Mice receiving daily CPI-444 in addition to adoptively transferred OT1 cells showed a significant improvement in tumor control and survival com- pared with vehicle-treated mice (Fig. 5a–c). CPI‑444 enhances T cell infiltration and effector function in an ACT model against B16‑OVA To further understand the specific role of A2aR signaling on the antitumor response in an ACT model, we were interested in interrogating antigen-specific T cell post-adoptive trans- fer. Mice with established B16-OVA tumors received ACT with activated Thy1.1-expressing OT1 T cells (Fig. 6a). Mice received either daily vehicle or CPI-444 by gavage. Animals were sacrificed and tissues were harvested 4 days after ACT. Despite a relatively short CPI-444 exposure time in this experiment, tumor weight measured at the time of harvest still demonstrated a measurable response to CPI- 444 treatment and ACT (Fig. 6b). Notably, we observed a significant increase in OT1 cell infiltration within tumors (Fig. 6c). OT1 cells in CPI-444-treated mice had markers of enhanced activation, demonstrated by increased expression of 41-BB, T-bet, and increased fraction of cells expressing CD69 (Fig. 6d–f). Of note, transferred OT1 T cells in CPI- 444-treated mice did not express significantly altered levels of PD-1 or LAG-3, or other checkpoint markers in tumors, lymph nodes, spleen, or blood compared with vehicle-treated control animals (Fig. 6g and data not shown). CD127 expres- sion was either unchanged or increased in CPI-444-treated mice (Fig. 6g, Supplemental Fig. 4). OT1 T cells from CPI- 444-treated mice showed significantly improved cytokine production (Fig. 6h, i). To assess the effect of A2aR block- ade on the endogenous T cell response simultaneously, we examined the Thy1.1 negative fraction of CD8+ CD44+ T cells. Consistent with Teff response observed in the CT26 model (Fig. 4), these endogenous cells showed decreased PD-1 and LAG-3 expression in dLNs, increased T-bet and 41-BB, and enhanced cytokine production upon PMA-iono- mycin stimulation (Supplemental Fig. 5). Overall, our stud- ies show that A2aR blockade during adoptive transfer results in enhanced antitumor response, with increased infiltration of adoptively transferred cells, as well as enhanced effector function within tumor tissue. Discussion As the field of immunotherapy in cancer has evolved, it has become evident that the metabolic characteristics of the tumor microenvironment present critical obstacles to fur- thering response rates [12, 27–31]. Several immunosuppres- sive metabolic pathways within in the TME are presently being targeted in early phase clinical trials, including agents that block IDO1, arginase1 (ARG1), and inducible nitric oxide synthase (iNOS). While nutrient depletion in the TME is an important source of immunosuppression, the accumula- tion of toxic metabolites, such as adenosine, is another cru- cial consequence of a dysregulated tumor metabolism that forms a significant hurdle for effective immunotherapy. Here we demonstrate that pharmacologic blockade of adenosine signaling through the A2a receptor with CPI-444 greatly enhances the efficacy of anti-PD-1 therapy in unmodified, syngeneic mouse tumor models and adoptive T cell therapy in an OVA-expressing melanoma model. Further, these studies reveal several fundamental mechanistic aspects of adenosine signaling in the context of immune cell interac- tions with cancer. Fig. 3 A2a receptor blockade with CPI-444 enhances efficacy of anti- PD-1 treatment in syngeneic tumors. a-f BALB/c (a–c) or C57BL/6 (d–f) mice were injected s.c. with 5 × 105 CT26 (a–c) or MC38 (d–f) tumor cells on day 0. Mice were treated with vehicle or CPI- 444 (100 mg/kg) on days 0–12 ± anti-PD-1 mAb on days 7, 9, 11, and 13. Time course of tumor growth presented as the mean tumor volume until the point of first animal sacrifice (a, d, left). Tumor volume compared on day of first sacrifice (a, d, right); spider plots depict tumor growth on individual mice (c, f). Error bars represent SEM. Survival data presented as Kaplan–Meier curve (b, e). Data shown are from a single experiment representative of three independ- ent experiments of n = 4–10 mice per group. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using two-tailed Mann–Whitney t test (a, d, right). We demonstrate a striking synergy between A2a receptor blockade with CPI-444 and anti-PD-1 treatment in synge- neic mouse tumor models (Fig. 3). It has previously been established that blockade of a single negative costimulatory pathway (e.g., PD-1), can trigger the expression of high levels of other unblocked checkpoint receptors that may curtail the function and expansion of responding immune cells [32, 33]. While this is a critical feature of several checkpoint pathways and should be considered as part of a rational basis for combination therapy, we demonstrate here that A2aR blockade with CPI-444 works in a signifi- cantly different manner, allowing the suppression of multiple other negative T cell co-receptors, especially LAG-3 and PD-1 (Figs. 1c–e, 2b, 4d–f and Supplemental Fig. 5a–c). In lowering the expression of checkpoint pathways on acti- vated CTLs, it is likely that A2aR blockade with CPI-444 effectively expands the pool of CTLs poised to respond to subsequent anti-PD-1 therapy (Supplemental Fig. 6). This is consistent with our observation of a relatively mild effect of A2aR blockade as monotherapy in tumor models, but significant synergistic effect with anti-PD-1 therapy (Fig. 3 and Supplemental Fig. 3). It is also consistent with previous reports showing that lower expression of PD-1 on T cells can lower the threshold for productive anti-PD-1 therapy [24]. It is important to note that, while T cells treated with CPI-444 expressed lower levels of these markers, this does not mean they are insufficiently activated. In fact, activated T cells in CPI-444 treated mice produce more cytokines upon restimulation, consistent with enhancement of an activated phenotype (Figs. 2c, d, 4i, 6h–I and Supplemental 5f–i). Fig. 4 A2a receptor blockade with CPI-444 modulates coinhibi- tory pathways and enhances effector function in the CT26 tumor model. a BALB/c mice were injected s.c. with 5 × 105 CT26 tumor cells in the right flank on day 0. Mice were treated with daily CPI- 444 (100 mg/kg) or vehicle on days 1–14 and sacrificed on day 14 for organ harvest. b–e CD44+ CD62L-CD8+Teff cells analyzed for expression of PD-1 and LAG-3 by flow cytometry in b tumor- infiltrating lymphocytes (TIL), c left inguinal non-draining lymph nodes (ndLN), d right inguinal draining lymph nodes (dLN), and e spleen. f–g Flow cytometry analysis of PD-1, LAG-3 (f) and FoxP3 (g), expressed by tumor-infiltrating FoxP3+ CD4+ regulatory T cells. h Flow cytometric analysis of T-bet and 41-BB expressed by tumor- infiltrating CD44+ CD8+ Teff cells. Data presented as representative MFI histograms (left) and summation data plots using combined data from two independent experiments of n = 4–6 mice per group. i Tumor-infiltrating CD8 T cells were analyzed by flow cytometry for cytokine expression after 4 h ex vivo stimulation with PMA/iono- mycin. Representative flow cytometry plots are shown. Fraction of CD8+ T cells producing IFNγ, TNFα, and fraction of double positive, IFNγ+TNFα+ CD8+ T cells are depicted in accompanying data sum- mary plots. Error bars represent SEM. Data are compiled from two independent experiments of n = 4–6 mice per group (b–g) or from a single experiment representative of at least three independent experi- ments of n = 4–6 mice per group (i). n.s. not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 using two-tailed Student’s t test. Fig. 5 A2aR blockade with CPI-444 enhances tumor growth sup- pression in a model of ACT. a–c Seven days after injection of 2 × 105 B16OVA tumor cells in the right flank C57BL/6 mice received 1.5 × 106 activated OT1 CD8 T cells. Mice were treated with daily CPI-444 (100 mg/kg) or vehicle by oral gavage on days 7–21. Time course of tumor growth presented as the mean tumor volume until the point of first animal sacrifice in OT1 treated groups (a, left). Tumor volume compared on day of first animal sacrifice (a, right). Survival data presented as Kaplan–Meier curve (b). Spider plots depict tumor growth on individual mice (c). Error bars represent SEM. Data shown are from a single experiment representative of three independent experiments of n = 4–10 mice per group. *p < 0.05 using two-tailed Mann–Whitney t test (a, left). Our studies in the CT26 tumor model strongly impli- cate the lymph node as a site of significant impact of A2aR blockade. Inasmuch as Teff activation during acute viral challenge occurs within activating lymph tissue, our vac- cinia-OVA experiments also strongly suggest the lymph node as a critical site of A2aR influence. Taken together, our experiments demonstrate that A2aR blockade dramatically influences Teff cells during initial priming and specifically at tumor-draining lymph nodes. These findings are particularly noteworthy in light of recent reports suggesting that PD-1 blockade has its most pronounced effects on CD28 signaling, rather than TCR signaling, suggesting that PD-1 signaling, such as A2aR signaling, is highly active during T cell acti- vation [34, 35]. In addition, other reports have specifically demonstrated that the antitumor effect of anti-PD-1 therapy requires CTL priming in the draining lymph node [36]. These reports in combination with our data, situate signifi- cant activity of both anti-PD-1 therapy and A2aR blockade at the draining lymph node. This anatomic co-localization at the draining lymph node in the setting of downregulated PD-1 on Teff cells in CPI-444 treated mice is likely the basis for the marked synergy apparent in our tumor models. These findings can have significant impact on translational studies, wherein the immune cell status within the draining lymph nodes of cancer patients may hold more predictive and cor- relative value than previously thought. The importance of the lymph node response in checkpoint regimens, such as those including CPI-444, could also have implications for clinical application of vaccines or other modalities specifically directed at T cell priming processes. Fig. 6 CPI-444 increases T cell infiltration and effector function in B16-OVA ACT model. (a) Ten days after B16OVA injection C57BL/6 mice received 1.5 × 106 activated congenically labeled Thy1.1+OT1 CD8 T cells. To ensure A2aR blockade upon trans- fer OT1 cells were activated in the presence of CPI-444 (5 μM) or vehicle. Mice were treated with daily CPI-444 (100 mg/kg) or vehicle by oral gavage on days 10–14 and sacrificed on day 14 for organ and tumor harvest. (b) Weight of harvested tumors. (c) Repre- sentative flow cytometry plots (above) depicting Thy1.1+OT1 cells recovered per infiltrating CD8+T cells in vehicle (left) and CPI-444 treated mice (right). Thy1.1+ CD8+ cell recovery data summary plots (below) per CD8+cells and per gram of tumor. (d) Representative flow cytometry plots depicting fraction of CD69+Thy1.1+OT1 cells with data summary plot (right). (e), (f) Representative flow cytom- etry histograms depicting MFI of 41-BB (e), T-bet (f), and data sum- mary plots for CD127 and PD-1 (g) expressed by Thy1.1+infiltrating CD8+ T cells. (h), (i) Thy1.1+OT1 T cells from tumors were ana- lyzed by flow cytometry for cytokine expression following ex vivo stimulation with SIINKEKL peptide. Representative flow cytometry plots are shown. Fraction of CD8+T cells producing IFNγ, TNFα, fraction of double positive IFNγ+TNFα+ (h) and IL-2 (i) CD8+ T cells are depicted in accompanying data summary plots. Error bars represent SEM. Data shown are from a single experiment representa- tive of three independent experiments of n = 4–9 mice per group. n.s. not significant, *p < 0.05, **p < 0.01 using two-tailed Student’s t test. While these findings imply that adenosine signaling is active within tumor-draining lymph nodes, the source of elevated adenosine within the lymph node is not clear. In this regard, it has been suggested that tumor derived exosomes expressing CD39/CD73 could have a significant influence on adenosine levels in draining lymph nodes [37, 38], and it has also been reported that metabolic characteristics of hypoxic regions of lymph nodes, such as germinal cent- ers, can elevate adenosine levels intrinsically [39, 40]. In addition, elevated circulating levels of adenosine derived from hypoxic tumor tissue could be contributing to elevated adenosine levels within draining lymph nodes. Of note, sys- temically elevated adenosine levels have been detected in patients with heart failure and in patients suffering from septic shock [41–44]. CD39/CD73 expressing cells within the lymph tissue itself, such as Tregs, could also be a source of locally generated adenosine. Overall, the effect of A2aR blockade during Teff cell priming is a novel finding of our work and implies that the most significant effects of adeno- sine blockade on effector cells may not be in the TME itself but by enhancing the immune response at proximal lymph nodes. The degree to which adenosine blockade at drain- ing lymph nodes is responsible for the improved effector function of tumor-infiltrating CTLs (Figs. 4i, 6h, i) versus a direct effect of A2aR blockade within the TME is unclear. In contrast to Teff cells, Tregs are well adapted to the metabolic milieu of TME. Activity of FoxP3 in Tregs has specifically been found to be a critical factor in this adapta- tion [45]. Transcriptional regulation of Tregs in response to hypoxia, adenosine and A2aR signaling has been an impor- tant subject in the field [46]. We show that adenosine A2aR blockade with CPI-444 suppresses the expression of FoxP3 on tumor-infiltrating Tregs (Fig. 4g). In addition, we pre- sent novel findings regarding the downregulation of several checkpoint pathways on FoxP3+ CD4+ regulatory T cells within the TME (Figs. 1f, 4f). While we did not observe significant reduction in intratumoral Treg numbers, to our knowledge this is the first description of multiple check- point pathways being downregulated on tumor-infiltrating Tregs in response to A2aR blockade. These findings are complementary to observations by Hatfield et al., demon- strating downregulation of FoxP3 and CTLA4 on Tregs in response to A2aR inhibition via hyperoxia [47]. While the precise role of these pathways in tumor-infiltrating Tregs are not well defined [25, 26, 48], their response to A2aR blockade is quite striking in our studies and deserves further investigation. Our findings showing increased effector function in adoptively transferred CD8+ T cells is in agreement with recently published studies of A2aR blockade in a CAR T cell model [49]. In our experiments, however, it is clear that A2aR blockade enhances the endogenous T cell response as well as adoptively transferred OT1 cells. Although suppressed expression of PD-1 and LAG-3 are observed in draining lymph nodes in these mice (Supplemental Fig. 5b, c), check- point expression on adoptively transferred OT1 cells is not affected by A2aR blockade (Fig. 6g). This implies, that while checkpoint modulation during priming is an important aspect of A2aR blockade, there is also a significant effect directly on effector function of adoptively transferred T cells (Fig. 6h, i). While increased infiltration of adoptively trans- ferred cells was not observed in previous studies using CAR T cells, it is likely that the differences between the CAR T cell model and our findings is related to distinct activa- tion pathways downstream of chimeric receptors and native TCRs. While OT1 cells in our ACT model require TCR and CD28 signaling during priming, CAR T cells are designed with intrinsic CD3 and CD28 signaling. This may be a criti- cal difference in the context of A2aR blockade. Understand- ing the precise basis for these differences will be informative and requires further study. As mentioned, previous studies have reported decreased T cell persistence in both A2aR-null mice and T cell-deleted A2aR mice [22, 23]. Using CPI-444 as pharmacologic blockade in our models has not caused a statistically sig- nificant decrease in expression of the early memory marker CD127 in response to vaccine or in ACT (Figs. 2a, 6g and Supplemental Figs. 2, 4a) and did not suppress memory response upon antigenic rechallenge (Fig. 2a). These find- ings demonstrate that, while persistent lack of A2aR signal- ing (as in germline knockout or conditional T cell knockout mice) has been shown to negatively affect the production of long-term memory, transient pharmacologic blockade of the A2aR within genetically unmodified, and syngeneic tumor models does not limit the viability of active Teff cells. More generally, these observations underscore the importance of validating observations from genetically modified mouse experiments in the setting of pharmacologic blockade. Overall, our studies lend significant evidence to the idea of the A2aR as a master regulator of anti-inflammatory path- ways, first put forth by Michail V. Sitkovsky and Akio Ohta over a decade ago [50]. In coordinately suppressing several checkpoint pathways on multiple T cell subsets critical to antitumor immunity, and with clinical trials underway, A2aR blockade has significant potential to broaden the armamen- tarium of cancer immunotherapy. Acknowledgements We thank members of the Powell lab, especially Chirag Patel, for critical discussion of the manuscript; Corvus phar- maceuticals for their generous gift of CPI-444; and Aduro Biotech for their generous gift of LM-OVA. Author contributions RDL designed and conducted the experiments and wrote the manuscript. I-MS, M-HO, I-HS, JW, and JE helped with the experiments. JDP designed the experiments and revised the manuscript. Funding This work was supported in part by funds from the Bloomberg~Kimmel Institute for Cancer Immunotherapy. In addition, CPI-444 and unrestricted research funds were provided by Corvus. Compliance with ethical standards Conflict of interest Jonathan D. Powell has been a paid consultant for Corvus and has equity in the company. All other authors declare that they have no conflicts of interest. Ethical approval and ethical standards All applicable international and national guidelines for the care of animals were followed. All mouse procedures approved by Johns Hopkins University Institutional Animal Care and Use Committee (Protocol #M016M103, approved 4/1/2016). Animal source C57BL/6 obtained from Charles River Laboratories (MC38 experiments) or Jackson Laboratories (ACT; 000664). OT-I and CD90.1, BALB/c mice obtained from The Jackson Laboratory. Male or female mice were used for each experiment; mice were sex and age matched accordingly. Cell line authentication MC38 cells were donated by CORVUS phar- maceuticals. The identity and specific pathogen free status of these cells was validated by microsatellite genotype analysis (IDEXX Biore- search). B16-OVA melanoma cells were a gift from Hyam Levitsky. All other tumor cell lines used were obtained from the ATCC. All cell lines were mycoplasma free via ELISA-based assays performed every 6 months. References 1. Ohta A, Sitkovsky M (2001) Role of G-protein-coupled adenosine receptors in downregulation of inflammation and protection from tissue damage. Nature 414:916–920 2. Ohta A, Gorelik E, Prasad SJ, Ronchese F, Lukashev D, Wong MK, Huang X, Caldwell S, Liu K, Smith P, Chen JF, Jackson EK, Apasov S, Abrams S, Sitkovsky M (2006) A2A adenosine receptor protects tumors from antitumor T cells. Proc Natl Acad Sci USA 103:13132–13137 3. Allard B, Pommey S, Smyth MJ, Stagg J (2013) Targeting CD73 enhances the antitumor activity of anti-PD-1 and anti-CTLA-4 mAbs. Clin Cancer Res 19:5626–5635 4. Beavis PA, Milenkovski N, Henderson MA, John LB, Allard B, Loi S, Kershaw MH, Stagg J, Darcy PK (2015) Adenosine receptor 2A blockade increases the efficacy of anti-PD-1 through enhanced anti-tumor T cell responses. Cancer Immunol Res 3:506–517 5. Waickman AT, Alme A, Senaldi L, Zarek PE, Horton M, Powell JD (2012) Enhancement of tumor immunotherapy by deletion of the A2A adenosine receptor. Cancer Immunol Immunother 61:917–926 6. Stagg J, Divisekera U, McLaughlin N, Sharkey J, Pommey S, Denoyer D, Dwyer KM, Smyth MJ (2010) Anti-CD73 antibody therapy inhibits breast tumor growth and metastasis. Proc Natl Acad Sci USA 107:1547–1552 7. Loi S, Pommey S, Haibe-Kains B, Beavis PA, Darcy PK, Smyth MJ, Stagg J (2013) CD73 promotes anthracycline resistance and poor prognosis in triple negative breast cancer. Proc Natl Acad Sci USA 110:11091–11096 8. Beavis PA, Divisekera U, Paget C, Chow MT, John LB, Devaud C, Dwyer K, Stagg J, Smyth MJ, Darcy PK (2013) Blockade of A2A receptors potently suppresses the metastasis of CD73 + tumors. Proc Natl Acad Sci USA 110:14711–14716 9. Mittal D, Young A, Stannard K, Yong M, Teng MW, Allard B, Stagg J, Smyth MJ (2014) Antimetastatic effects of blocking PD-1 and the adenosine A2A receptor. Cancer Res 74:3652–3658 10. Zarek PE, Huang CT, Lutz ER, Kowalski J, Horton MR, Linden J, Drake CG, Powell JD (2008) A2A receptor signaling pro- motes peripheral tolerance by inducing T-cell anergy and the generation of adaptive regulatory T cells. Blood 111:251–259 11. Parks SK, Cormerais Y, Pouysségur J (2017) Hypoxia and cel- lular metabolism in tumour pathophysiology. J Physiol (Lond) 595:2439–2450 12. Kishton RJ, Sukumar M, Restifo NP (2017) Metabolic regula- tion of T cell longevity and function in tumor immunotherapy. Cell Metab 26:94–109 13. Blay J, White TD, Hoskin DW (1997) The extracellular fluid of solid carcinomas contains immunosuppressive concentrations of adenosine. Cancer Res 57:2602–2605 14. Dubyak GR, el-Moatassim C (1993) Signal transduction via P2-purinergic receptors for extracellular ATP and other nucleo- tides. Am J Physiol 265:C577–C606 15. Robeva AS, Woodard RL, Jin X, Gao Z, Bhattacharya S, Tay- lor HE, Rosin DL, Linden J (1996) Molecular characterization of recombinant human adenosine receptors. Drug Dev Res 39:243–252 16. Apasov S, Koshiba M, Redegeld F, Sitkovsky MV (1995) Role of extracellular ATP and P1 and P2 classes of purinergic recep- tors in T-cell development and cytotoxic T lymphocyte effector functions. Immunol Rev 146:5–19 17. Apasov SG, Koshiba M, Chused TM, Sitkovsky MV (1997) Effects of extracellular ATP and adenosine on different thy- mocyte subsets: possible role of ATP-gated channels and G protein-coupled purinergic receptor. J Immunol 158:5095–5105 18. Filippini A, Taffs RE, Agui T, Sitkovsky MV (1990) Ecto- ATPase activity in cytolytic T-lymphocytes. Protection from the cytolytic effects of extracellular ATP. J Biol Chem 265:334–340 19. Resta R, Yamashita Y, Thompson LF (1998) Ecto-enzyme and signaling functions of lymphocyte CD73. Immunol Rev 161:95–109 20. Emens L, Powderly J, Fong L, Brody J, Forde P, Hellmann M, Hughes B, Kummar S, Loi S, Luke J, Mahadevan D, Mark- man B, McCaffery I, Miller R, Laport G (2017) CPI-444, an oral adenosine A2a receptor (A2aR) antagonist, demonstrates clinical activity in patients with advanced solid tumors. AACR Annual Meeting 2017. Cancer Res 77:Abstract CT119 21. Willingham S, Ho P, Leone R, Piccione E, Choy C, Hotson A, Buggy J, Powell J, Miller R (2016) The adenosine A2A receptor antagonist CPI-444 blocks adenosine-mediated T-cell suppres- sion and exhibits antitumor activity alone and in combination with anti-PD-1 and anti-PD-L1. AACR Annual Meeting 2017. Cancer Res 76:Abstract 2337 22. Cekic C, Sag D, Day YJ, Linden J (2013) Extracellular adeno- sine regulates naive T cell development and peripheral mainte- nance. J Exp Med 210:2693–2706 23. Cekic C, Linden J (2014) Adenosine A2A receptors intrinsically regulate CD8+ T cells in the tumor microenvironment. Cancer Res 74:7239–7249 24. Ngiow SF, Young A, Jacquelot N, Yamazaki T, Enot D, Zitvo- gel L, Smyth MJ (2015) A threshold level of intratumor CD8+ T-cell PD1 expression dictates therapeutic response to anti-PD1. Cancer Res 75:3800–3811 25. Chaudhary B, Elkord E (2016) Regulatory T Cells in the tumor microenvironment and cancer progression: role and therapeutic targeting. Vaccines (Basel). https://doi.org/10.3390/vaccines40 30028 26. Huang CT, Workman CJ, Flies D, Pan X, Marson AL, Zhou G, Hipkiss EL, Ravi S, Kowalski J, Levitsky HI, Powell JD, Pardoll DM, Drake CG, Vignali DA (2004) Role of LAG-3 in regulatory T cells. Immunity 21:503–513 27. Ohta A (2016) A metabolic immune checkpoint: adenosine in tumor microenvironment. Front Immunol 7:109 28. Smyth MJ, Ngiow SF, Ribas A, Teng MWL (2016) Combination cancer immunotherapies tailored to the tumour microenvironment. Nat Rev Clin Oncol 13:143–158 29. Jiang S, Yan W (2016) T-cell immunometabolism against cancer. Cancer Lett 382:255–258 30. Mockler MB, Conroy MJ, Lysaght J (2014) Targeting T cell immunometabolism for cancer immunotherapy; understanding the impact of the tumor microenvironment. Front Oncol 4:107 31. Siska PJ, Rathmell JC (2015) T cell metabolic fitness in antitumor immunity. Trends Immunol 36:257–264 32. Curran MA, Montalvo W, Yagita H, Allison JP (2010) PD-1 and CTLA-4 combination blockade expands infiltrating T cells and reduces regulatory T and myeloid cells within B16 melanoma tumors. Proc Natl Acad Sci USA 107:4275–4280 33. Koyama S, Akbay EA, Li YY, Herter-Sprie G, Buczkowski KA, Richards WG, Gandhi L, Redig AJ, Rodig SJ, Asahina H, Jones RE, Kulkarni MM, Kuraguchi M, Palakurthi S, Fecci PE, Johnson BE, Janne PA, Engelman JA, Gangadharan SP, Costa DB, Free- man GJ, Bueno R, Hodi FS, Dranoff G, Wong K, Hammerman PS (2016) Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints. Nat Commun 7:10501 34. Kamphorst AO, Wieland A, Nasti T, Yang S, Zhang R, Barber DL, Konieczny BT, Daugherty CZ, Koenig L, Yu K, Sica GL, Sharpe AH, Freeman GJ, Blazar BR, Turka LA, Owonikoko TK, Pillai RN, Ramalingam SS, Araki K, Ahmed R (2017) Rescue of exhausted CD8 T cells by PD-1-targeted therapies is CD28- dependent. Science 355:1423–1427 35. Hui E, Cheung J, Zhu J, Su X, Taylor MJ, Wallweber HA, Sas- mal DK, Huang J, Kim JM, Mellman I, Vale RD (2017) T cell costimulatory receptor CD28 is a primary target for PD-1-medi- ated inhibition. Science 355:1428–1433 36. Chamoto K, Chowdhury PS, Kumar A, Sonomura K, Matsuda F, Fagarasan S, Honjo T (2017) Mitochondrial activation chemicals synergize with surface receptor PD-1 blockade for T cell-depend- ent antitumor activity. Proc Natl Acad Sci USA 114:E770 37. Vijayan D, Young A, Teng MWL, Smyth MJ (2017) Target- ing immunosuppressive adenosine in cancer. Nat Rev Cancer 17:709–724 38. Smyth LA, Ratnasothy K, Tsang JYS, Boardman D, Warley A, Lechler R, Lombardi G (2013) CD73 expression on extracellular vesicles derived from CD4+ CD25+ Foxp3+ T cells contributes to their regulatory function. Eur J Immunol 43:2430–2440 39. Abbott RK, Thayer M, Labuda J, Silva M, Philbrook P, Cain DW, Kojima H, Hatfield S, Sethumadhavan S, Ohta A, Reinherz EL, Kelsoe G, Sitkovsky M (2016) Germinal center hypoxia poten- tiates immunoglobulin class switch recombination. J Immunol 197:4014–4020 40. Ohta A, Diwanji R, Kini R, Subramanian M, Ohta A, Sitkovsky M (2011) In vivo T cell activation in lymphoid tissues is inhibited in the oxygen-poor microenvironment. Front Immunol 2:27 41. Martin C, Leone M, Viviand X, Ayem ML, Guieu R (2000) High adenosine plasma concentration as a prognostic index for outcome in patients with septic shock. Crit Care Med 28:3198–3202 42. Funaya H, Kitakaze M, Node K, Minamino T, Komamura K, Hori M (1997) Plasma adenosine levels increase in patients with chronic heart failure. Circulation 95:1363–1365 43. Vallon V, Miracle C, Thomson S (2008) Adenosine and kidney function: potential implications in patients with heart failure. Eur J Heart Fail 10:176–187 44. Ramakers BPC, Riksen NP, Broek PHH, Franke B, Peters WHM, Hoeven JG, Smits P, Pickkers P (2011) Circulating adenosine increases during human experimental endotoxemia but blockade of its receptor does not influence the immune response and sub- sequent organ injury. Crit Care 15:R3 45. Angelin A, Gil-de-Gómez L, Dahiya S, Jiao J, Guo L, Levine MH, Wang Z, Quinn WJ, Kopinski PK, Wang L, Akimova T, Liu Y, Bhatti TR, Han R, Laskin BL, Baur JA, Blair IA, Wallace DC, Hancock WW, Beier UH (2017) Foxp3 reprograms T cell metabo- lism to function in low-glucose, high-lactate environments. Cell Metab 25:1282–1293.e7 46. Sitkovsky MV (2009) T regulatory cells: hypoxia-adenosinergic suppression and re-direction of the immune response. Trends Immunol 30:102–108 47. Hatfield SM, Kjaergaard J, Lukashev D, Schreiber TH, Belikoff B, Abbott R, Sethumadhavan S, Philbrook P, Ko K, Cannici R, Thayer M, Rodig S, Kutok JL, Jackson EK, Karger B, Podack ER, Ohta A, Sitkovsky MV (2015) Immunological mechanisms of the antitumor effects of supplemental oxygenation. Sci Transl Med 7:277ra30 48. Whiteside TL (2014) Induced regulatory T cells in inhibitory microenvironments created by cancer. Expert Opin Biol Ther 14:1411–1425 49. Beavis PA, Henderson MA, Giuffrida L, Mills JK, Sek K, Cross RS, Davenport AJ, John LB, Mardiana S, Slaney CY, Johnstone RW, Trapani JA, Stagg J, Loi S, Kats L, Gyorki D, Kershaw MH, Darcy PK (2017) Targeting the adenosine 2A receptor enhances chimeric antigen receptor T cell efficacy. J Clin Invest 127:929–941 50. Sitkovsky MV, Ohta A (2005) The ‘danger’ sensors that STOP Ciforadenant the immune response: the A2 adenosine receptors? Trends Immunol 26:299–304.